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Updated: Aug 29, 2025
Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications
Published on: April 13, 2022
Preparation of nanochitin hydrogel with adjustable inter-structure by sequencial genipin crosslinking and
Feier Chen1, Ying Liu1, Yujun Zou1
1Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Abstract:
In this work, genipin crosslinked chitin nanofibril hydrogels/cryogels were successfully prepared. Due to the acidic disperse nature of positively charged chitin fibrils, the pH nature for the chemical crosslinking between chitin nanofibrils and genipin was restricted under 5.0. The pH nature and crosslinking temperature were found greatly influence the efficient chemical crosslinking between chitin nanofibril and genipin. Further ice templated crosslinking was found effective enhancing the mechanical properties of resulting chitin nanofibril hydrogels/cryogels. The cryogel prepared by genipin-crosslinked DEChNs showed interconnected pores, presenting a state of stacking layers. In addition, the deposition of hydroxyapatite (HA) on the surface of chitin nanofibril scaffolds was found effective for improving chondrocyte attachments and growths. The cells viability indicated the good cell compatibility of the cryogel. All the results indicated that the cryogel prepared by genipin-crosslinked DEChNs showed a potential for tissue engineering applications.
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