Denitrification performance, bioelectricity generation and microbial response in microbial fuel cell - constructed...

For the deep reduction of nitrogen, the microbial fuel cell-constructed wetland (MFC-CW) was conducted for treating carbon constraint wastewater. Results indicated that nitrogen removal decreased from 94.96% to 24.96% with influent COD/TN (chemical oxygen demand/total nitrogen) from 4 to 0. MFC-CW was seriously affected by low organic wastewater. Wetland plants contributed to denitrification, with TN removal increasing from 46.13% to 64.87%. The bioenergy output showed a linear relationship with influent COD, and the maximum power density of 1.17 mW/m² was obtained. Correlation analysis indicated that functional genera of Paenibacillus, Trichococcus, norank_KD4-96, norank_OLB14 played a crucial role in nitrogen removal. Influent COD and wetland plants affected carbon and nitrogen metabolisms, and key genes related to denitrification were more abundant in the cathode. Findings illustrated the nitrogen metabolism in MFC-CW with carbon constraint wastewater and will extend the application of MFC-CW in secondary effluent treatment from wastewater treatment plants.