A new technique for separating platelet-rich plasma by a copolymer device － without a centrifugation process

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Biotechnology

Background:

• Platelet-rich plasma (PRP) has broad clinical utility.
• Current commercial PRP preparation often relies on centrifugation.

Purpose of the Study:

• To develop a novel, non-centrifugation-based PRP preparation device.
• To evaluate the efficacy of a cationic copolymer-coated polyurethane sponge for PRP isolation.

Main Methods:

• A polyurethane sponge was coated with a novel cationic copolymer.
• The copolymer's cytotoxicity and hemolysis were assessed.
• PRP preparation efficiency was compared to a commercial kit (RegenKit-THT).

Main Results:

• The copolymer coating demonstrated no cytotoxicity or hemolysis.
• PRP derived from the device showed high platelet and angiogenic growth factor concentrations.
• The isolated PRP promoted cell migration, angiogenesis, and matrix metalloproteinase gene expression.

Conclusions:

• A cationic copolymer-coated polyurethane sponge serves as an effective PRP preparation device.
• This method eliminates the need for centrifugation in PRP preparation.
• The developed device offers a promising alternative for clinical PRP applications.