Behavior and Functional Roles of CD34⁺ Mesenchymal Cells in Mammalian Testes

Mammalian testes consist of seminiferous tubules within which Sertoli cells line up at the periphery and nurse germ cells, and of interstitia that harbor various cells such as peritubular myoid cells (PMCs), Leydig cells (LCs), vascular endothelial cells, immune cells such as macrophages, and mesenchymal (stromal) cells. Morphological studies have recently reported the presence of telocytes with telopodes in the interstitium of adult mouse, rat, and human testes. CD34⁺PDGFRα⁺ telocytes with long and moniliform telopodes form reticular networks with various cell types such as LCs, PMCs, and vessels, indicating their potential functions in cell-cell communications and tissue homeostasis. Functional studies have recently been performed on testicular interstitial cells and CD34⁺ cells, using 3D re-aggregate cultures of dissociated testicular cells, and cell cultures. Direct observation of CD34⁺ cells and adult LCs (ALCs) revealed that CD34⁺ cells extend thin cytoplasmic processes (telopodes), move toward the LC-CD34⁺ cell-re-aggregates, and finally enter into the re-aggregates, indicating the chemotactic behavior of CD34⁺ telocytes toward ALCs. In mammalian testes, important roles of mesenchymal interstitial cells as stem/progenitors in the differentiation and regeneration of LCs have been reported. Here, reports on testicular telocytes so far obtained are reviewed, and future perspectives on the studies of testicular telocytes are noted.