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Transcriptome Dynamics Underlying Magnesium Deficiency Stress in Three Founding Saccharum Species
Yongjun Wang1, Yihan Li1, Xiuting Hua2
1Center for Genomics and Biotechnology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Key Laboratory of Sugarcane Biology and Genetic Breeding, National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Abstract:
Modern sugarcane cultivars were generated through interspecific crossing of the stress resistance Saccharum spontaneum and the high sugar content Saccharum officinarum which was domesticated from Saccharum robustum. Magnesium deficiency (MGD) is particularly prominent in tropical and subtropical regions where sugarcane is grown, but the response mechanism to MGD in sugarcane remains unknown. Physiological and transcriptomic analysis of the three founding Saccharum species under different magnesium (Mg) levels was performed. Our result showed that MGD decreased chlorophyll content and photosynthetic efficiency of three Saccharum species but led to increased starch in leaves and lignin content in roots of Saccharum and Saccharum . We identified 12,129, 11,306 and 12,178 differentially expressed genes (DEGs) of Saccharum , Saccharum and Saccharum , respectively. In Saccharum , MGD affected signal transduction by up-regulating the expression of xylan biosynthesis process-related genes. Saccharum responded to the MGD by regulating the expression of transcription and detoxification process-related genes. Saccharum , avoids damage from MGD by regulating the expression of the signing transduction process and the transformation from growth and development to reproductive development. This novel repertoire of candidate genes related to MGD response in sugarcane will be helpful for engineering MGD tolerant varieties.
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Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
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