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Structural Characteristics of the 5'-Terminal Region of Mouse p53 mRNA and Identification of Proteins That Bind to

Joanna Szpotkowska1,2, Kamil Szpotkowski1, Jerzy Ciesiołka1

  • 1Institute of Bioorganic Chemistry, Polish Academy of Sciences, 61-704 Poznan, Poland.

International Journal of Molecular Sciences
|September 9, 2022
PubMed

Insights

Researchers explored the 5'-terminal region of mouse p53 mRNA, revealing its structure and protein interactions critical for p53 protein synthesis regulation. Key proteins like hnRNP K and PCBP2 bind this region, influencing gene expression.

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Gene Expression Regulation

Background:

  • Mouse models are crucial for studying p53 gene expression.
  • Understanding p53 mRNA structure and protein interactions is vital for interpreting functional studies.
  • The 5'-terminal region of mouse p53 mRNA regulates p53 protein and its isoform Δ41p53 synthesis.

Purpose of the Study:

  • To elucidate the spatial folding of the 5'-terminal region of mouse p53 mRNA.
  • To identify proteins binding to this critical mRNA region.
  • To map protein binding sites and their functional implications.

Main Methods:

  • Small-angle X-ray scattering (SAXS) for structural analysis.
  • RNAComposer for predicting RNA spatial folding.
  • RNA-assisted affinity chromatography to identify binding proteins.
  • Oligonucleotide hybridization assays to map accessible sites.

Main Results:

  • The spatial structure of the 5'-terminal region of mouse p53 mRNA was determined.
  • Proteins binding to the 5'-terminal region were identified from mouse fibroblast lysates.
  • hnRNP K and PCBP2 showed high binding affinity to the 5'-terminal region.
  • Identified protein binding sites often overlap with accessible regions for oligonucleotide hybridization.

Conclusions:

  • The 5'-terminal region of mouse p53 mRNA possesses a defined structure influencing protein synthesis.
  • hnRNP K and PCBP2 are key proteins interacting with this mRNA region.
  • Structural insights into mRNA-protein interactions are essential for understanding p53 regulation.
Keywords:
5′ non-coding regionPCBP2RNA structureRNA-assisted affinity chromatographyhnRNP Kmouse p53 mRNA

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