Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

176
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
176
CRISPR01:59

CRISPR

52.7K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.7K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.3K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.1K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.1K
RNA Editing02:23

RNA Editing

9.1K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hormesis induced by lithium exposures influenced the effects of maternal age at reproduction on offspring quality of Brachionus calyciflorus: A multigenerational study.

Aquatic toxicology (Amsterdam, Netherlands)·2026
Same author

A spatially confined "double-key lock" smart DNA hydrogel for dynamic detection of MicroRNA in cells.

Chemical science·2026
Same author

Development of Peroxymonosulfate Catalytic System and Photoenzymatic Catalytic System Utilizing Zn-Doped g-C<sub>3</sub>N<sub>4</sub> for the Degradation of Methylene Blue in Water.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

A High-Performance Solid-State Secondary Battery Using a Triple-Phase-Interface Anode Displaying Excellent Capacity and Electrochemical Kinetics.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Integrating Protein Language Models with Multimodal Embeddings to Accelerate Function Prediction of Uncharacterized Proteins.

International journal of molecular sciences·2026
Same author

Enabling High-Performance Lithium Metal Batteries by Stabilizing the Anode Interface with a Trace 6FDA Additive.

ACS applied materials & interfaces·2026

Related Experiment Video

Updated: Aug 29, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.3K

Prime Editing: An All-Rounder for Genome Editing.

Chenyu Lu1, Jingyu Kuang1, Tong Shao1

  • 1Department of Biology and Chemistry, College of Sciences, National University of Defense Technology, Changsha 410073, China.

International Journal of Molecular Sciences
|September 9, 2022
PubMed
Summary

Prime editing (PE) offers versatile "search-and-replace" genome editing. Optimizations are enhancing PE efficiency and delivery, overcoming barriers for broader applications.

Keywords:
applicationsgenome editingprime editingtechnical optimization

More Related Videos

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
07:31

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Published on: February 17, 2023

1.2K
Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
07:17

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

3.2K

Related Experiment Videos

Last Updated: Aug 29, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.3K
Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
07:31

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Published on: February 17, 2023

1.2K
Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
07:17

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

3.2K

Area of Science:

  • Genetics
  • Molecular Biology
  • Biotechnology

Background:

  • Prime editing (PE) is an advanced genome editing technology with a "search-and-replace" mechanism.
  • PE offers versatile editing capabilities, potentially surpassing existing genome editing technologies in scope.
  • Despite its potential, PE faces challenges in efficiency, specificity, and delivery.

Purpose of the Study:

  • To review the development and functional principles of prime editing technology.
  • To identify key barriers hindering the broader application of PE.
  • To discuss current optimization efforts and future application directions for PE.

Main Methods:

  • Literature review of prime editing technology development.
  • Analysis of prime editing system optimizations.
  • Discussion of application scope, potential risks, and future research directions.

Main Results:

  • Prime editing demonstrates superior all-in-one genome editing potential.
  • Key challenges include improving editing efficiency, reducing off-target effects, and developing effective delivery systems.
  • Ongoing optimizations are enhancing PE performance and expanding its application prospects.

Conclusions:

  • Prime editing is a powerful genome editing tool with significant potential.
  • Addressing current limitations is crucial for its widespread adoption in various fields.
  • Further research is needed to fully realize the capabilities of PE technology.