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Updated: Aug 29, 2025

Multi-modal Imaging of Angiogenesis in a Nude Rat Model of Breast Cancer Bone Metastasis Using Magnetic Resonance Imaging, Volumetric Computed Tomography and Ultrasound

Multi-modal Imaging of Angiogenesis in a Nude Rat Model of Breast Cancer Bone Metastasis Using Magnetic Resonance Imaging, Volumetric Computed Tomography and Ultrasound

Published on: August 14, 2012

Understanding Breast Cancers through Spatial and High-Resolution Visualization Using Imaging Technologies.

Haruko Takahashi¹, Daisuke Kawahara², Yutaka Kikuchi¹

¹Graduate School of Integrated Sciences for Life, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8526, Hiroshima, Japan.

|September 9, 2022

Summary

This review explores 3D imaging techniques for breast cancer tissue, focusing on tissue clearing and optical methods. It highlights how understanding tissue structure aids in analyzing cancer progression and gene expression.

Keywords:

optical three-dimensional imaging photoacoustic imaging spatial transcriptomics tissue clearing

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Last Updated: Aug 29, 2025

Multi-modal Imaging of Angiogenesis in a Nude Rat Model of Breast Cancer Bone Metastasis Using Magnetic Resonance Imaging, Volumetric Computed Tomography and Ultrasound

Multi-modal Imaging of Angiogenesis in a Nude Rat Model of Breast Cancer Bone Metastasis Using Magnetic Resonance Imaging, Volumetric Computed Tomography and Ultrasound

Published on: August 14, 2012

Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer

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Published on: March 24, 2013

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

Area of Science:

Oncology
Biomedical Imaging
Molecular Biology

Background:

Breast cancer is a leading global health concern for women.
Traditional breast cancer research often overlooks the complex tissue microenvironment.
Recent advancements necessitate studying the 3D architecture of breast cancer tissues.

Purpose of the Study:

To review methods for analyzing the 3D structure of breast cancer tissue.
To highlight technological advances in tissue clearing and 3D optical imaging.
To explore the link between 3D cell arrangement, gene expression, and breast cancer malignancy.

Main Methods:

Tissue clearing techniques for enhanced optical penetration.
Optical 3D imaging for high-resolution structural analysis.
Integration of gene expression analysis with 3D spatial data.
Noninvasive photoacoustic imaging for in vivo characterization.

Main Results:

Tissue clearing combined with optical 3D imaging offers unprecedented insights into breast cancer tissue architecture.
3D spatial arrangement of cells correlates with malignant transformation and metastasis.
Gene expression patterns can be mapped onto the 3D tissue structure.
Photoacoustic imaging provides a noninvasive window into 3D breast cancer tissue features.

Conclusions:

Advanced 3D imaging methods are crucial for a comprehensive understanding of breast cancer.
Analyzing the 3D tumor microenvironment is key to deciphering cancer progression and metastasis.
Integrating structural and molecular data offers new avenues for breast cancer research and potential therapeutic strategies.