Related Experiment Video
Updated: Aug 29, 2025
07:38
Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants
Published on: June 6, 2025
232
Novel UHRF1-MYC Axis in Acute Lymphoblastic Leukemia
Soyoung Park1, Ali H Abdel Sater1, Johannes F Fahrmann1
1Department of Clinical Cancer Prevention, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
Cancers
|September 9, 2022
Summary
Overexpression of UHRF1 protein is found in acute lymphoblastic leukemia (ALL). Targeting UHRF1 may offer a new therapeutic strategy for ALL by impacting c-Myc expression.
Area of Science:
- Oncology
- Molecular Biology
- Hematology
Background:
- Ubiquitin-like, containing PHD and RING finger domain (UHRF) proteins are implicated as oncogenes.
- UHRF family members' role in acute leukemia requires further investigation.
Purpose of the Study:
- To evaluate UHRF family protein abundance in acute leukemia.
- To investigate the functional role of UHRF1 in ALL pathogenesis.
Main Methods:
- Protein abundance analysis in leukemia patient samples.
- Transcriptomic dataset analysis.
- In-vitro siRNA-mediated knockdown of UHRF1.
- Mechanistic studies on UHRF1-c-Myc interaction and downstream pathways.
Main Results:
- UHRF1 protein is significantly overexpressed in ALL compared to AML, CLL, and CML.
- UHRF1 knockdown in B-ALL and T-ALL cell lines reduced cell viability and c-Myc expression.
- UHRF1 directly interacts with c-Myc, promoting ALL expansion through the CDK4/6-phosphoRb pathway.
Conclusions:
- UHRF1 plays a critical role in ALL pathogenesis by regulating c-Myc.
- UHRF1 represents a potential therapeutic target for acute lymphoblastic leukemia.
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