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Related Experiment Video

Updated: Aug 29, 2025

Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water
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Colorimetric Approach for Nucleic Acid Salmonella spp. Detection: A Systematic Review.

Asma Nadia Ahmad Faris1, Mohamad Ahmad Najib1, Muhammad Najmi Mohd Nazri1

  • 1Institute for Research in Molecular Medicine (INFORMM), Health Campus, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia.

International Journal of Environmental Research and Public Health
|September 9, 2022
PubMed
Summary

Colorimetric nucleic acid methods offer a rapid and simple way to detect food-poisoning bacteria like Salmonella. Further research is needed to confirm sensitivity and specificity for routine diagnostic use.

Keywords:
Salmonellacolorimetricdetectionnucleic acidsystematic review

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Area of Science:

  • Food safety and microbiology
  • Biosensing technologies
  • Molecular diagnostics

Background:

  • Foodborne illnesses caused by bacteria such as Salmonella pose significant public health risks.
  • Current detection methods for these pathogens are often slow and labor-intensive.
  • Colorimetric strategies present a promising alternative for rapid and accurate bacterial sensing.

Purpose of the Study:

  • To review advancements in colorimetric nucleic acid-based methods for Salmonella detection.
  • To synthesize findings from recent literature on this diagnostic approach.

Main Methods:

  • A systematic literature search was performed across PubMed, Scopus, and ScienceDirect.
  • Fifteen relevant studies out of 88 identified were selected for analysis.
  • The review focused on colorimetric nucleic acid assays for identifying various Salmonella species.

Main Results:

  • The colorimetric method successfully detected multiple Salmonella species, including S. Typhimurium, S. Enteritidis, S. Typhi, and S. Paratyphi A.
  • Limit of Detection (LoD) was assessed using colony-forming units (CFU) and CFU per mL.
  • Spiked samples were more commonly used (53%) than real samples (33%) to determine LoDs.

Conclusions:

  • Colorimetric nucleic acid detection shows potential for simple, rapid Salmonella identification.
  • Further investigation is required to fully evaluate the sensitivity and specificity of these methods for routine diagnostics.
  • The application of colorimetric assays in clinical and food safety settings warrants additional research.