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Updated: Aug 20, 2026

Measuring Fast Calcium Fluxes in Cardiomyocytes
Published on: November 29, 2011
A comparison between 45Ca2+ and atomic absorption in calcium flux determinations in perfused rat liver
Abstract:
Calcium efflux from perfused rat liver following the administration of Ca2+ releasing agents was measured with two methods, 45Ca2+ labeling and atomic absorption. The values obtained with atomic absorption were usually higher than the values obtained with 45Ca2+. These indicate that the intracellular Ca2+ did not equilibrate with the perfusate ca2+ during the 90-minute labeling period. A similar conclusion was reached by measuring the liver 45Ca2+ and 40Ca2+ content. In addition, the types of albumin added to the perfusate influenced the amounts of Ca2+ released.
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