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Updated: Aug 29, 2025

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Nano-Ag Particles Embedded in C-Matrix: Preparation, Properties and Application in Cell Metabolism.

Sylwia Terpilowska1, Stanislaw Gluszek1, Elzbieta Czerwosz2

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Materials (Basel, Switzerland)
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This study explores silver nanograins in a carbon matrix, finding that their size impacts cytotoxicity, genotoxicity, and mutagenicity. This research is crucial for developing safer nanomaterials for medical applications.

Keywords:
cytotoxicitygenotoxicitymutagenicitynanocomposite nano-Ag

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Toxicology

Background:

  • Silver nanoparticles (nano-Ag) have historical applications as antimicrobial agents.
  • Toxicity concerns arise from bulk or large-sized silver materials.
  • Understanding nano-Ag's cellular effects is vital for safe medical use.

Purpose of the Study:

  • To investigate the influence of nano-Ag grains embedded in a carbonaceous matrix on cytotoxicity, genotoxicity, and mutagenicity.
  • To assess the impact of varying nano-Ag grain sizes on these toxicological endpoints.

Main Methods:

  • Fabrication of a nanocomposite film using Physical Vapor Deposition (PVD) from fullerene and silver acetate sources.
  • Characterization of the material's structure and morphology via SEM/EDX, XRD, and Raman spectroscopy.
  • Toxicological evaluation of materials with different nano-Ag grain sizes on fibroblasts.

Main Results:

  • The study successfully prepared silver nanograins stabilized within a carbonaceous matrix.
  • Toxicology studies revealed that cell viability, genotoxicity, and mutagenicity are dependent on the size of the embedded Ag nanograins.
  • The carbonaceous matrix plays a role in stabilizing the silver nanograins.

Conclusions:

  • The size of silver nanograins significantly influences their toxicological profile.
  • Developing controlled nano-Ag grain sizes within a carbon matrix is key to mitigating toxicity.
  • This research provides insights for designing safer nano-Ag based materials for biomedical applications.