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High-throughput Screening and Biosensing with Fluorescent C. elegans Strains
Published on: May 19, 2011
A novel fluorescence biosensor based on CRISPR/Cas12a integrated MXenes for detecting Aflatoxin B1
Zhihui Wu1, Da-Wen Sun2, Hongbin Pu1
1School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, & Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China.
A novel fluorescence biosensor utilizing CRISPR/Cas12a and MXenes offers rapid, sensitive detection of Aflatoxin B1 (AFB1) in food. This advancement addresses global food safety concerns by providing a reliable method for quantifying AFB1 contamination.
Area of Science:
- Food Science
- Biotechnology
- Analytical Chemistry
Background:
- Aflatoxin B1 (AFB1) contamination poses a significant threat to global food safety.
- Rapid and quantitative detection methods for AFB1 are crucial but remain challenging.
Purpose of the Study:
- To develop a novel fluorescence biosensor for the sensitive and rapid detection of AFB1.
- To leverage CRISPR/Cas12a and MXenes for enhanced AFB1 quantification.
Main Methods:
- A fluorescence biosensor was designed using dual-AFB1 aptamers, CRISPR/Cas12a, and MXenes.
- MXenes adsorbed ssDNA-FAM, quenching fluorescence; AFB1 binding released an activator, triggering Cas12a trans-cleavage and fluorescence recovery.
Main Results:
- The biosensor exhibited a wide detection range (0.001–80 ng/mL) and a low detection limit (0.92 pg/mL).
- Detection was achieved within 80 minutes, demonstrating high sensitivity and speed.
- The platform showed excellent performance in detecting AFB1 in real peanut samples.
Conclusions:
- The developed CRISPR/Cas12a-MXene fluorescence biosensor provides a promising tool for rapid and quantitative AFB1 detection.
- This method enhances food safety by enabling efficient monitoring of AFB1 contamination in food products.
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