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Analytical systems ranked by freedom from interferences.

M R Glick, K W Ryder

    Clinical Chemistry
    |August 1, 1987
    PubMed
    Summary
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    Hemolysis, lipemia, and bilirubinemia significantly impact clinical chemistry results. Analyzers with physical barriers or protein separation, and those using appropriate blanking, show fewer interferences from these common specimen interferences.

    Area of Science:

    • Clinical Chemistry
    • Analytical Toxicology
    • Laboratory Medicine

    Background:

    • Specimen quality is crucial for accurate clinical chemistry testing.
    • Common pre-analytical interferences include hemolysis, lipemia, and bilirubinemia.
    • These interferences can affect the performance of various analytical systems.

    Purpose of the Study:

    • To quantitatively assess the impact of hemolysis, lipemia, and bilirubinemia on 22 common clinical chemistry analyzers.
    • To rank analytical systems based on their susceptibility to these common interferences.
    • To identify analyzer design features and methods that mitigate interference.

    Main Methods:

    • Standardized serum specimens were prepared with known levels of hemolysis, lipemia, and bilirubinemia.

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  • Interference was evaluated across 22 different clinical chemistry analytical systems.
  • A quantitative rating scheme was developed based on observed interferences relative to the number of methods evaluated.

    • Main Results:

    • Significant variability in interference effects was observed across the evaluated analytical systems.
    • Systems incorporating physical barriers or protein-separation steps generally performed better.
    • Direct analyzers using appropriate blanking wavelengths, particularly specimen blank absorbance, showed fewer interferences.

    Conclusions:

    • The choice of analytical system significantly influences the reliability of clinical chemistry results in the presence of common interferences.
    • Analyzer design, including physical barriers and protein separation, is key to minimizing interference.
    • Effective blanking strategies are essential for direct analyzers to ensure accurate results.