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Updated: Aug 29, 2025
Use of In vivo Imaging to Monitor the Progression of Experimental Mouse Cytomegalovirus Infection in Neonates
Published on: July 6, 2013
Mouse Models for Cytomegalovirus Infections in Newborns and Adults
Ilija Brizić1, Berislav Lisnić1, Fran Krstanović1
1Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
Abstract:
This article describes procedures for infecting adult mice with murine cytomegalovirus (MCMV) and for infecting newborn mice to model congenital CMV infection. Methods are included for propagating MCMV in cell cultures and preparing a more virulent form of MCMV from the salivary glands of infected mice. A plaque assay is provided for determining MCMV titers of infected tissues or virus stocks. Also, methods are described for preparing the murine embryonic fibroblasts used for propagating MCMV, and for the plaque assay. © 2022 Wiley Periodicals LLC.
Insights
This study details methods for infecting mice with murine cytomegalovirus (MCMV) to model congenital CMV infection. Procedures cover virus propagation, virulence enhancement, and titer determination using plaque assays for research applications.
Area of Science:
- Virology
- Infectious Diseases
- Animal Models
Background:
- Murine cytomegalovirus (MCMV) is a model pathogen for studying human cytomegalovirus (hCMV) infection.
- Congenital cytomegalovirus (CMV) infection is a significant public health concern, necessitating robust animal models for research.
Purpose of the Study:
- To provide standardized protocols for MCMV infection in mice.
- To establish methods for generating virulent MCMV strains and quantifying viral loads.
- To facilitate research into CMV pathogenesis and potential therapeutic strategies.
Main Methods:
- Infection of adult and newborn mice with MCMV.
- Propagation of MCMV in murine embryonic fibroblast (MEF) cell cultures.
- Preparation of a high-titer, virulent MCMV stock from infected mouse salivary glands.
- Quantification of MCMV titers using a plaque assay in MEFs.
Main Results:
- Established protocols for successful MCMV infection in both adult and newborn mouse models.
- Demonstrated methods for MCMV propagation and preparation of virulent stocks.
- Validated a plaque assay for accurate MCMV titer determination in various samples.
Conclusions:
- The described procedures offer a comprehensive toolkit for MCMV research.
- These methods support the study of MCMV infection dynamics and congenital CMV disease modeling.
- Standardized protocols enhance reproducibility and comparability in CMV research.
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