Related Experiment Video
Updated: Aug 29, 2025
13:37
Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library
Published on: June 20, 2014
18.3K
One Bead-One Compound (OBOC) Peptidomimetic-Encoded Library Synthesis via Split-and-Pool Methods.
Claudio Aquino1, Mohosin Sarkar2
1Neuron23, Inc, South San Francisco, CA, USA. claudioaquino525@gmail.com.
Methods in Molecular Biology (Clifton, N.J.)
|September 9, 2022
Summary
We developed a straightforward method for creating diverse peptidomimetic compound libraries. This technique enables the synthesis of large one-bead-one-compound libraries for drug discovery and chemical biology applications.
Area of Science:
- Chemical Biology
- Drug Discovery
- Medicinal Chemistry
Background:
- Peptidomimetic libraries are crucial for identifying high-affinity compounds.
- Existing methods can be complex for generating diverse libraries.
Purpose of the Study:
- To present a simple and general method for synthesizing large peptidomimetic libraries.
- To enable the creation of structurally diverse one-bead-one-compound (OBOC) libraries.
Main Methods:
- Submonomer solid-phase synthesis approach.
- Generation of one-bead-one-compound (OBOC) libraries.
- Encoding of compounds by mass or genetic methods.
Main Results:
- Successful synthesis of large, structurally diverse peptidomimetic libraries.
- Demonstration of a versatile and scalable synthetic strategy.
- Compatibility with mass and genetic encoding techniques.
Conclusions:
- The described method offers a powerful tool for chemical biology and drug discovery.
- Facilitates the rapid identification of novel therapeutic agents.
- Enables the generation of diverse compound libraries for screening.
Keywords:
One bead-one compound (OBOC) librarySolid phase synthesisSplit-and-poolSubmonomer synthesis
