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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Updated: Aug 29, 2025

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
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Next Generation Sequencing of DNA-Encoded Libraries.

Jian Liu1, You Li2

  • 1HitGen Inc., Chengdu, Sichuan, China. jian.liu@hitgen.com.

Methods in Molecular Biology (Clifton, N.J.)
|September 9, 2022
PubMed
Summary

This study presents an optimized method for sequencing DNA Enrichment Libraries (DEL). The customized protocol enhances process efficiency and improves DNA sequencing quality on Illumina platforms.

Keywords:
Affinity selectionDNA-encoded library (DEL)Illumina sequencingNext generation sequencingPreparative PCR

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • DNA Enrichment (DEL) technology relies heavily on extensive DNA sequencing data.
  • Current Illumina sequencing library preparation involves multiple steps, including PCR, end repair, adenylation, adapter ligation, and a second PCR.

Purpose of the Study:

  • To describe an improved method for sequencing DEL libraries.
  • To enhance the efficiency and quality of DEL library preparation and sequencing.

Main Methods:

  • Utilized a customized preparative PCR protocol for sequencing DEL libraries.
  • Integrated standard purification, analysis, and DNA sequencing steps on an Illumina platform.

Main Results:

  • The customized method significantly improved process efficiency compared to standard protocols.
  • Achieved enhanced sequencing quality with the described approach.

Conclusions:

  • The presented method offers a more efficient and higher-quality alternative for DEL library sequencing.
  • This optimization is crucial for the analysis of large DNA sequence datasets in DEL technology.