EXPERIMENTAL EVALUATION OF DISTRIBUTION OF 14C PHOTOASSIMILATED INTO CARBOHYDRATES IN DIFFERENT GROWTH STAGES OF
Yuhi Satoh1, Shogo Imada1, Yasuhiro Tako1
1Department of Rasioecology, Institute for Environmental Sciences (IES), 1-7 Rokkasho, Aomori 039-3212, Japan.
Abstract:
In this study, we aimed to investigate the photoassimilation process of 14CO2 into agricultural plants through determining the photoassimilated carbohydrate-13C in each part (leaves, current branch and fruit) of the fruit-bearing apple shoots exposed to 13CO2 in different growth stages (early and late fruit development stages). The carbohydrate content was assessed as soluble (ethanol-extracted fraction) and other (HCl-extracted and residual fractions) components. The total (i.e. sum of the three fractions) bulk carbohydrate concentrations in all parts of the shoots were statistically similar between different growth stages. The changes in the concentration of 13C-labeled soluble carbohydrate (i.e. ethanol-extracted fraction) to the total content between different growth stages were statistically unclear among all parts of the shoot. These results suggest that the distribution ratio of photoassimilated 13C in soluble and other components in the apple shoot was thereabout constantly independent of the growth stages.
