Animal model testing for efficacy and adverse effects of bacteriophages against Acinetobacter baumannii
Aamir Hussain1, Ihsan Ullah1, Muhammad Q Saeed2
1Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan.
Abstract:
This study aimed to conduct a detailed study on murine model testing of bacteriophage against Acinetobacter baumannii. These bacteriophages were tested not only for their efficacy in healing wound of murine models infected with multidrug resistant A. baumannii but were also studied for any derangement in hematological parameters as well as liver and kidney function. The study also included any histological changes observed in hepatic and renal tissues of the bacteriophage treated murine animals. This experimental study was conducted at Institute of Basic Medical Sciences, Khyber Medical University, Peshawar and Institute of Pure and Applied Biology, Department of Microbiology and Molecular Genetics, Bahauddin Zakariya University, Multan. A. baumannii isolates were obtained from the Microbiology Department, Armed Forces Institute of Pathology, Rawalpindi. Antimicrobial susceptibility was done by using standard procedures and as per Clinical Laboratory Standards Institute guidelines. Bacteriophages were isolated from sewage water samples collected from different hospitals in Multan. These bacteriophages were characterized and finally used for treating the murine model and efficacy of phage as a therapeutic option was determined by using superficial rat wound model. In this study, the isolated lytic bacteriophage was effective in relatively faster wound healing of the infected animals. Moreover, there were no significant hematological or renal and hepatic profile changes in treated animals. Histology of renal and hepatic tissues was also normal as compared to control animals. Our study concluded that the isolated phage could serve as an attractive therapeutic candidate to combat the deadly multidrug resistant A. baumannii.
Insights
Bacteriophages effectively healed wounds in a murine model infected with multidrug-resistant Acinetobacter baumannii without causing adverse effects. This phage therapy shows promise for treating A. baumannii infections.
Area of Science:
- Microbiology
- Infectious Diseases
- Therapeutic Development
Background:
- Multidrug-resistant Acinetobacter baumannii poses a significant threat in healthcare settings.
- Limited treatment options exist for Acinetobacter baumannii infections.
- Bacteriophage therapy offers a potential alternative to antibiotics.
Purpose of the Study:
- To evaluate the efficacy of isolated bacteriophages in treating Acinetobacter baumannii wound infections in a murine model.
- To assess the safety profile of bacteriophage therapy by examining hematological parameters and organ function.
- To investigate histological changes in hepatic and renal tissues following bacteriophage treatment.
Main Methods:
- Isolation and characterization of lytic bacteriophages from sewage water samples.
- In vitro susceptibility testing of Acinetobacter baumannii isolates.
- Treatment of superficial rat wound models infected with multidrug-resistant Acinetobacter baumannii using bacteriophages.
- Monitoring of hematological parameters, liver and kidney function tests.
- Histopathological examination of liver and kidney tissues.
Main Results:
- Isolated bacteriophages demonstrated effectiveness in promoting faster wound healing in infected murine models.
- No significant adverse effects were observed in hematological parameters, liver function, or kidney function of treated animals.
- Histological examination revealed normal hepatic and renal tissues in bacteriophage-treated animals compared to controls.
Conclusions:
- The isolated bacteriophage is a safe and effective therapeutic candidate for combating multidrug-resistant Acinetobacter baumannii infections.
- Bacteriophage therapy presents a promising alternative to conventional antibiotics for Acinetobacter baumannii infections.
- Further research into bacteriophage therapy for Acinetobacter baumannii is warranted.
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