Physical memory of astrocytes

Traumatic brain injury (TBI) is a major risk factor for development of neurodegenerative disorders later in life. Short, repetitive, mechanical impacts can lead to pathology that appears days or months later. The cells have a physical "memory" of mechanical events. The origin of this memory is not known. To examine the properties of this memory, we used a microfluidic chip to apply programmed fluid shear pulses to adherent adult rat astrocytes. These caused a transient rise in intracellular Ca²⁺. In response to repeated stimuli, 6 to 24 hrs apart, the Ca²⁺ response increased. This effect lasted longer than 24 hrs. The Ca²⁺ responses were more sensitive to the number of repetitions than to the rest time between stimuli. We found that inhibiting the Ca²⁺ influx during conditioning stimulus did not eliminate the stress potentiation, suggesting that mechanical deformation during the primary injury is accountable for the later response. The mechanical mechanism that triggers this long term "memory" may act by plastic deformation of the cytoskeleton.