Development of Compact Readout Device for Neural Observation System using Fluorescence Imaging and Fast-scan Cyclic...

Area of Science:

• Neuroscience
• Biomedical Engineering
• Sensor Technology

Background:

• Simultaneous neural observation and neurochemical recording are crucial for understanding brain function.
• Existing systems often require multiple devices or complex setups, limiting their applicability in freely behaving subjects.

Purpose of the Study:

• To develop a compact, dual-functional readout device for simultaneous neural imaging and neurotransmitter detection.
• To enable simpler, in-vivo recording of neural activity and dopamine levels in freely behaving animals.

Main Methods:

• Integration of an implantable CMOS image sensor and a fast-scan cyclic voltammetry (FSCV) system.
• Implementation on a microcontroller-based platform for a compact and portable device.
• Sequential operation of imaging and voltammetry functions with optimized parameters.

Main Results:

• The device achieves a maximum imaging rate of 160 fps with a 120x268 pixel sensor.
• Voltammetry function verified using commercial carbon fiber electrodes.
• System operates at up to 60 fps when sequentially performing imaging with FSCV at 400 V/s scan rate.
• Successful simultaneous calcium imaging and dopamine recording in freely behaving mice.

Conclusions:

• The developed readout device offers a simplified approach for dual-function neural monitoring.
• This technology serves as a foundation for future implantable multi-functional neural sensors.
• The system enhances the ability to study neural circuits and neurochemical dynamics in real-time.