Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Special Staining Techniques01:13

Special Staining Techniques

223
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
223
Flagella and Motility in Bacteria01:18

Flagella and Motility in Bacteria

307
Flagella are specialized, thread-like structures that extend from a bacteria's cell envelope. They play a crucial role in motility and chemotaxis. Their structural organization and functioning exemplify sophisticated biological engineering, enabling bacterial survival and adaptability in diverse environments.Structure of the FlagellumA bacterial flagellum consists of three key components: the filament, the hook, and basal body. The filament, a long, helical structure composed of repeating...
307
Classification of Skeletal Muscle Fibers01:48

Classification of Skeletal Muscle Fibers

56.8K
Skeletal muscles continuously produce ATP to provide the energy that enables muscle contractions. Skeletal muscle fibers can be categorized into three types based on differences in their contraction speed and how they produce ATP, as well as physical differences related to these factors. Most human muscles contain all three muscle fiber types, albeit in varying proportions.
Slow-Twitch Muscle Fibers
Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...
56.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PSF-Driven Spatio-Temporal Blending in Fluorescence Lifetime Imaging Microscopy and Its Mitigation via Mean-Shift Super-Resolution-Based Masking.

bioRxiv : the preprint server for biology·2026
Same author

A discontinuously coupled network of phase oscillators replicates cooperation between motors in actomyosin systems.

Journal of the Royal Society, Interface·2026
Same author

3D+t human sperm flagellum centerline dataset.

Scientific data·2026
Same author

Chloride Intracellular Channel 4 (CLIC4) controls volume regulation in sperm development via Protein Kinase C (PKC).

BMC biology·2026
Same author

Thermal modulation of CatSper activity regulates hyperactivated motility in human sperm†.

Biology of reproduction·2026
Same author

Automatic Segmentation of the Left Ventricle Through the Cardiac Cycle in Pediatric Echocardiography Videos Using SegFormer Architecture.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025
Same journal

Analysis of End-Tidal CO2 Variability During Plateau Waves Episodes: An Information Theoretic Approach<sup></sup>.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025
Same journal

AI and Tomosynthesis for Breast Cancer Molecular Subtyping: A step toward precision medicine<sup></sup>.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025
Same journal

Towards Sustainable Protein Recovery from Biological Waste: Assessing Polyethersulfone-based Microfiltration.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025
Same journal

Analysis of the cardiovascular response to standardized polymicrobial peritonitis experimental model.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025
Same journal

Automated Wrist Ultrasound Image Bone Enhancement and Segmentation Using Deep Learning.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025
Same journal

A Deep Learning approach for Depressive Symptoms assessment in Parkinson's disease patients using facial videos.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025
See all related articles

Related Experiment Video

Updated: Aug 29, 2025

Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract
10:07

Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract

Published on: June 6, 2019

12.4K

3D+t feature-based descriptor for unsupervised flagellar human sperm beat classification.

Haydee O Hernandez, Paul Hernandez-Herrera, Fernando Montoya

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
    |September 10, 2022
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed a new 3D method to analyze human sperm motility, classifying hyperactivated sperm based on flagellar movement. This technique offers a promising tool for assessing sperm viability and fertilization potential.

    More Related Videos

    Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
    08:48

    Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

    Published on: April 21, 2022

    2.6K
    Biophysical Characterization of Flagellar Motor Functions
    06:08

    Biophysical Characterization of Flagellar Motor Functions

    Published on: January 18, 2017

    8.3K

    Related Experiment Videos

    Last Updated: Aug 29, 2025

    Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract
    10:07

    Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract

    Published on: June 6, 2019

    12.4K
    Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
    08:48

    Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

    Published on: April 21, 2022

    2.6K
    Biophysical Characterization of Flagellar Motor Functions
    06:08

    Biophysical Characterization of Flagellar Motor Functions

    Published on: January 18, 2017

    8.3K

    Area of Science:

    • Reproductive Biology
    • Biophysics
    • Sperm Motility Analysis

    Background:

    • Human sperm capacitation is crucial for fertilization, involving motility changes.
    • Traditional 2D sperm motility analysis is limited; 3D analysis is more representative of natural environments.
    • Characterizing 3D hyperactivated sperm motility is challenging due to high flagellar beat frequencies and lack of visual ground truth.

    Purpose of the Study:

    • To characterize the three-dimensional (3D) motility patterns of hyperactivated human spermatozoa.
    • To develop an unsupervised method for classifying sperm based on 3D flagellar motion.
    • To establish a novel tool for assessing sperm fertilization viability.

    Main Methods:

    • Utilized a 3D+t image acquisition system to track sperm flagella.
    • Proposed a 3D feature vector using ellipses to describe the spatio-temporal envelope of flagellar motion.
    • Employed unsupervised hierarchical clustering to classify sperm motility patterns.

    Main Results:

    • Successfully separated hyperactivated sperm from non-capacitated cells using the 3D feature vector and clustering.
    • Identified three main clusters of flagellar motility patterns.
    • Validated the 3D method by correlating its principal component with 2D motility analysis (correlation coefficient ~0.7).

    Conclusions:

    • The proposed 3D+t feature-based descriptor provides an effective method for unsupervised classification of human sperm motility.
    • This novel approach offers a promising tool for determining sperm viability and potential for egg fertilization.
    • Advances the understanding of sperm capacitation and hyperactivation in a 3D context.