Examining the Impact of Sample Thickness Variations on the Hyperspectral Radiometric Responses of Flowing Blood

The hyperspectral reflectance and transmittance of flowing blood samples are employed in a wide range of biomedical applied research initiatives such as the detection and monitoring of hematological abnormalities. The success of these initiatives is tied to the correct interpretation of these radiometric quantities. This, in turn, requires a comprehensive understanding about their sensitivity to variations in the experimental conditions in which they have been obtained. In this paper, we aim to contribute to these efforts by systematically examining the effects of sample thickness variations on these quantities. More specifically, we employed controlled in silico experiments to assess these effects on samples with different biophysical characteristics, notably their hematocrit, hemolysis level and orientation of their constituent cells with respect to the flow direction. To ensure a high degree of fidelity in our experiments, we used a first-principles simulation framework supported by measured data. Our findings unveil distinct spectrally-dependent trends associated with reflectance and transmittance changes elicited by sample thickness variations.