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Chromatin-associated microprocessor assembly is regulated by the U1 snRNP auxiliary protein PRP40

Agata Stepien1, Jakub Dolata1, Tomasz Gulanicz1

  • 1Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznan 61-614, Poland.

The Plant Cell
|September 10, 2022
PubMed

Insights

Arabidopsis PRE-MRNA PROCESSING PROTEIN 40 (PRP40) protein is crucial for microRNA (miRNA) biogenesis. It helps assemble the microprocessor complex on miRNA genes, ensuring proper miRNA production.

Area of Science:

  • Plant molecular biology
  • Gene regulation
  • RNA processing

Background:

  • MicroRNA (miRNA) biogenesis in plants involves cotranscriptional processing by the microprocessor complex.
  • The microprocessor complex processes primary miRNA transcripts generated by RNA polymerase II (RNAPII).

Purpose of the Study:

  • To investigate the role of Arabidopsis PRE-MRNA PROCESSING PROTEIN 40 (AtPRP40) in miRNA biogenesis.
  • To elucidate how AtPRP40 influences the assembly and function of the plant microprocessor complex.

Main Methods:

  • Analysis of prp40ab mutant plants.
  • Chromatin immunoprecipitation to assess protein-DNA associations.
  • Monitoring of RNAPII and miRNA precursor localization at transcription sites.

Main Results:

  • AtPRP40 positively regulates the recruitment of SERRATE to miRNA genes.
  • Mutations in PRP40 altered DICER-LIKE1 (DCL1) association with chromatin.
  • Impaired microprocessor assembly led to RNAPII accumulation and precursor retention at miRNA genes in prp40ab mutants.

Conclusions:

  • AtPRP40 is essential for efficient cotranscriptional microprocessor assembly at miRNA genes.
  • This regulation by AtPRP40 impacts RNAPII transcription and ensures correct miRNA levels.
  • AtPRP40 plays a key role in plant miRNA biogenesis and gene regulation.

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