Related Experiment Video
Updated: Aug 29, 2025
07:31
ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
Published on: June 30, 2022
2.5K
Chromatin-associated microprocessor assembly is regulated by the U1 snRNP auxiliary protein PRP40
Agata Stepien1, Jakub Dolata1, Tomasz Gulanicz1
1Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznan 61-614, Poland.
The Plant Cell
|September 10, 2022
Summary
Arabidopsis PRE-MRNA PROCESSING PROTEIN 40 (PRP40) protein is crucial for microRNA (miRNA) biogenesis. It helps assemble the microprocessor complex on miRNA genes, ensuring proper miRNA production.
Area of Science:
- Plant molecular biology
- Gene regulation
- RNA processing
Background:
- MicroRNA (miRNA) biogenesis in plants involves cotranscriptional processing by the microprocessor complex.
- The microprocessor complex processes primary miRNA transcripts generated by RNA polymerase II (RNAPII).
Purpose of the Study:
- To investigate the role of Arabidopsis PRE-MRNA PROCESSING PROTEIN 40 (AtPRP40) in miRNA biogenesis.
- To elucidate how AtPRP40 influences the assembly and function of the plant microprocessor complex.
Main Methods:
- Analysis of prp40ab mutant plants.
- Chromatin immunoprecipitation to assess protein-DNA associations.
- Monitoring of RNAPII and miRNA precursor localization at transcription sites.
Main Results:
- AtPRP40 positively regulates the recruitment of SERRATE to miRNA genes.
- Mutations in PRP40 altered DICER-LIKE1 (DCL1) association with chromatin.
- Impaired microprocessor assembly led to RNAPII accumulation and precursor retention at miRNA genes in prp40ab mutants.
Conclusions:
- AtPRP40 is essential for efficient cotranscriptional microprocessor assembly at miRNA genes.
- This regulation by AtPRP40 impacts RNAPII transcription and ensures correct miRNA levels.
- AtPRP40 plays a key role in plant miRNA biogenesis and gene regulation.
Related Concept Videos
02:41Chromatin Structure Regulates pre-mRNA Processing
7.1K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
7.1K
01:45Regulation of Nuclear Protein Sorting
2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
02:25Spreading of Chromatin Modifications
8.5K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
Writers
The writer...
8.5K
02:36RNA Polymerase II Accessory Proteins
9.4K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.4K
02:54Nucleosome Remodeling
9.4K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
9.4K
01:31Regulation of the Unfolded Protein Response
2.5K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.5K