Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long-term real-world outcomes following preoperative radiotherapy and conservative surgery for trunk and extremity soft tissue sarcomas: a retrospective cohort study.

EClinicalMedicine·2026
Same author

Simulation-Free Single-Fraction High-Dose Radiotherapy for Non-Spine Bone and Soft Tissue Metastases.

Practical radiation oncology·2026
Same author

Low muscle mass predicts liver-related outcomes in cirrhosis, independent of portal hypertension: A retrospective cohort study.

Canadian liver journal·2026
Same author

Adaptive Radiation Therapy in the 0-7-21 Regimen: A Retrospective Study of Tumor Volumetric Response.

Advances in radiation oncology·2026
Same author

Impact of a Multidisciplinary Palliative Radiation Therapy Clinic on Patient Care.

Advances in radiation oncology·2026
Same author

Efficacy and safety of metastasis-directed therapy for oligometastatic cancer: a systematic review and meta-analysis of randomized controlled trials.

Radiation oncology (London, England)·2026

Related Experiment Video

Updated: Aug 29, 2025

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro
16:18

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Published on: November 20, 2011

11.7K

Cell Death Analysis in Cancer Spheroids from a Microfluidic Device.

Julie Lafontaine1, Elena Refet-Mollof1,2, Ouafa Najyb1

  • 1Institut du Cancer de Montréal (ICM), Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montreal, QC, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|September 10, 2022
PubMed
Summary

Microfluidic devices enable cost-effective, high-throughput drug screening using 3D cancer spheroids. This approach aids in discovering novel drug combinations for cancer radiotherapy enhancement.

Keywords:
3D cultureAnnexin VChemotherapyClonogenic assayDrug screeningImageJLab-on-chipMicrofluidicRadiotherapySpheroids

More Related Videos

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
10:33

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

Published on: June 16, 2019

28.0K
Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery
13:19

Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery

Published on: April 26, 2024

2.9K

Related Experiment Videos

Last Updated: Aug 29, 2025

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro
16:18

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Published on: November 20, 2011

11.7K
Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
10:33

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

Published on: June 16, 2019

28.0K
Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery
13:19

Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery

Published on: April 26, 2024

2.9K

Area of Science:

  • Oncology
  • Biotechnology
  • Drug Discovery

Background:

  • 3D cancer spheroids offer a more relevant preclinical model than 2D cultures.
  • Microfluidic technology allows for precise control and miniaturization of biological assays.
  • Combination therapies, including radiotherapy, are crucial for overcoming cancer treatment resistance.

Purpose of the Study:

  • To develop and validate a microfluidic system for cytotoxicity evaluation of cancer spheroids.
  • To identify potential drug combinations that enhance the efficacy of radiotherapy.
  • To establish a cost-effective platform for preclinical drug screening.

Main Methods:

  • Fabrication and preparation of microfluidic devices for spheroid culture.
  • Seeding of cancer cells to form 3D spheroids within the microfluidic device.
  • Cytotoxicity assessment using spheroid size, colony formation assays, and flow cytometry for apoptotic markers (Annexin V).

Main Results:

  • Successful generation and culture of cancer spheroids in microfluidic devices.
  • Demonstration of microfluidic system's capability for high-throughput drug screening.
  • Quantification of cell death and apoptotic responses to various treatments.

Conclusions:

  • Microfluidic-based spheroid models provide a powerful tool for preclinical cancer drug discovery.
  • This system facilitates the identification of synergistic drug combinations for radiotherapy.
  • The technology enables low-cost, efficient evaluation of therapeutic strategies.