Apoptosis Detection by Quantification of Cell Debris in Bright-Field Microscopy Images

We describe a simple protocol for quantification of apoptosis by counting subcellular debris particles in bright-field microscopy images of cell culture media samples. The only necessary equipment is a bright-field microscope (fitted with a digital camera) and a computer for image processing and analysis. The method gives comparable results to established fluorescence markers in several different applications and is, in principle, compatible with any culture format. Given its simplicity, accessibility, and inexpensive nature, the method can complement or provide an alternative to other methods for apoptosis detection.