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Updated: Aug 6, 2026

Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
Published on: August 30, 2018
Therapeutic drug monitoring in a pediatric laboratory
Abstract:
Therapeutic drug monitoring using a new and faster high pressure liquid chromatographic (HPLC) method is discussed. Analysis of chloramphenicol and succinate esters and a large number of anticonvulsive drugs can be carried out using the same buffer and reverse-phase HPLC column by changing the solvent concentration to reflect the choice of drugs. A buffer prepared from 0.02 M sodium acetate and trifluoroacetic acid provides an optimal mobile phase for separation of a wide variety of drugs commonly used in pediatric medicine. Sample preparation and the use of special internal standards without extensive extractions provides excellent accuracy, reliability and sensitivity, and requires a minimum of preparation.
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