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MiRNA-144-5p down-modulates CDCA3 to regulate proliferation and apoptosis of lung adenocarcinoma cells

Jing Luo1, Lilong Xia1, Lei Zhang1

  • 1Thoracic Surgery, Zhejiang Hospital, Hangzhou, Zhejiang Province 310000, China.

Mutation Research
|September 10, 2022
PubMed
Abstract

Insights

MicroRNA-144-5p inhibits lung adenocarcinoma (LUAD) growth by targeting CDCA3. Overexpression of this microRNA suppresses LUAD cell proliferation and promotes apoptosis, offering potential therapeutic insights.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Lung adenocarcinoma (LUAD) is a major non-small cell lung cancer subtype with limited treatment options and poor survival rates.
  • MicroRNAs (miRNAs) play crucial roles in cancer development and progression.
  • Current treatments like chemotherapy and radiotherapy have significant side effects and low efficacy.

Purpose of the Study:

  • To investigate the molecular mechanism of miRNA-144-5p in regulating lung adenocarcinoma proliferation.
  • To identify CDCA3 as a direct target of miRNA-144-5p in LUAD.
  • To elucidate the role of the miRNA-144-5p/CDCA3 axis in LUAD cell cycle regulation and apoptosis.

Main Methods:

  • Differential expression analysis of miRNA and mRNA data from the TCGA-LUAD dataset.
  • Bioinformatic prediction and dual-luciferase reporter assay to validate miRNA-target interactions.
  • Functional assays including CCK-8, colony formation, spheroidization, and flow cytometry to assess LUAD cell behavior.
  • Western blot analysis to evaluate protein expression levels of CDCA3, p53, and cell cycle markers.

Main Results:

  • MiRNA-144-5p was significantly downregulated in LUAD tissues.
  • Overexpression of miRNA-144-5p inhibited LUAD cell proliferation and spheroidization while promoting apoptosis.
  • MiRNA-144-5p directly targets CDCA3, and this interaction regulates LUAD cell proliferation.
  • The miRNA-144-5p/CDCA3 pathway influences LUAD cell cycle progression and apoptosis, involving the p53 signaling pathway.

Conclusions:

  • MiRNA-144-5p acts as a tumor suppressor in LUAD by targeting CDCA3.
  • The miRNA-144-5p/CDCA3 axis plays a critical role in regulating LUAD cell proliferation, apoptosis, and cell cycle.
  • Understanding this mechanism provides insights into LUAD pathogenesis and potential therapeutic strategies.
Keywords:
ApoptosisCDCA3Lung adenocarcinomaMiRNA-144–5pP53Proliferation

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