Structural enrichment and identification of lignin-carbohydrate complex in alkaline stabilized system

Effectively breaking down its anti-depolymerization barrier is key to the conversion and utilization of biomass resources. The anti-depolymerization barrier is mainly composed of a lignin-carbohydrate complex (LCC), which hinder the high-value utilization of biomass. In an alkaline environment, phenyl glycosidic bonds in LCC exist stably. Therefore, it is of great significance for the structural analysis of LCC in alkaline environment. In this study, LCC rich in carbohydrates was extracted from alkaline hemicellulose by using the xylanase-macroporous resin method, the effects of enzyme dosage and treatment time on LCC enrichment were discussed. The results showed that the LC bond of LCC samples was dominated by phenyl glucoside bond, the composition of lignin showed the rule of S > H > G. This method could fulfill the requirements of lignin enrichment and structural integrity. This provides a new method for solving the problems of LCC signal masking and low detection sensitivity.