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Updated: Aug 29, 2025
Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
Published on: June 5, 2021
Permissive omicron breakthrough infections in individuals with binding or neutralizing antibodies to ancestral
Erin Williams1, Jordan Colson2, Ranjini Valiathan2
1Department of Otolaryngology, University of Miami Miller School of Medicine, Miami, FL 33136, USA; Department of Biomedical Engineering, University of Miami, Miami, FL 33136, USA.
Background:
Breakthrough infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant (B.1.1.529) has occurred in populations with high vaccination rates.
Methods:
In a longitudinal cohort study, pre-breakthrough infection sera for Omicron breakthroughs (n = 12) were analyzed. Assays utilized include a laboratory-developed solid phase binding assay to recombinant spike protein, a commercial assay to the S1 domain of the spike protein calibrated to the World Health Organization (WHO) standard, and a commercial solid-phase surrogate neutralizing activity (SNA) assay. All assays employed spike protein preparations based on sequences from the Wuhan-Hu-1 strain.
Results:
Pre-breakthrough binding antibody titers ranged from 1:800 to 1:51,200 for the laboratory-developed binding assay, which correlated well and agreed quantitatively with the commercial spike S1 domain WHO calibrated assay. SNA was detected in 10/12 (83%) samples.
Conclusions:
Neither high binding titers nor SNA were markers of protection from Omicron infection/re-infection.
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