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Genome Characteristic of Bordetella parapertussis Isolated from Iran

Azadeh Safarchi1,2, Samaneh Saedi3, Chin Yen Tay4

  • 1School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia. a.safarchi@unsw.edu.au.

Current Microbiology
|September 10, 2022
PubMed

Insights

Bordetella parapertussis, a cause of whooping cough, was identified in Iran. Genomic analysis revealed evolutionary changes in an infant isolate, highlighting ongoing bacterial adaptation.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Pertussis (whooping cough) is a respiratory infection primarily caused by Bordetella pertussis, but Bordetella parapertussis can cause similar symptoms, particularly severe in infants.
  • Bordetella parapertussis infections are often underdiagnosed or misdiagnosed as B. pertussis.

Purpose of the Study:

  • To identify Bordetella parapertussis isolates from clinical samples in Iran.
  • To investigate the virulence factor expression and genomic characteristics of B. parapertussis.
  • To understand the evolutionary dynamics of B. parapertussis.

Main Methods:

  • Bacterial culture and biochemical tests for isolate identification.
  • IS1001 insertion detection for confirmation.
  • Western blot analysis for pertactin (Prn) expression.
  • Whole-genome sequencing (Illumina NextSeq) of an infant isolate (IRBP134).
  • Comparative genomics and phylogenetic analysis.

Main Results:

  • Seven Bordetella parapertussis isolates were identified from nasal swabs in Iran between 2012-2015.
  • The isolate IRBP134 exhibited a G+C content of 65% and a genome size of 4.7 Mbp.
  • Genomic comparison revealed 81 single nucleotide polymorphisms and 13 insertions/deletions compared to the reference genome, indicating evolutionary changes.
  • Phylogenetic analysis placed IRBP134 within the global B. parapertussis population.

Conclusions:

  • Bordetella parapertussis is circulating in Iran and can cause pertussis-like illness.
  • Genomic analysis demonstrates ongoing evolution and genetic variation within B. parapertussis populations.
  • Further research is needed to understand the clinical significance and public health impact of B. parapertussis.

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