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Updated: Aug 29, 2025

Author Spotlight: Employing Green-Chemistry Principles for Safe and Sustainable Synthesis of Biodiesels
Published on: April 19, 2024
Obtention of biodiesel through an enzymatic two-step process. Study of its performance and characteristic emissions
Mariana Macías-Alonso1, Rosa Hernández-Soto1, Marcelino Carrera-Rodríguez1
1Instituto Politécnico Nacional, Unidad Profesional Interdisciplinaria de Ingeniería Campus Guanajuato Av. Mineral de Valenciana 200 Col. Fracc. Industrial Puerto Interior Silao 36275 Guanajuato Mexico jgonzalezm@ipn.mx mcarrerar@ipn.mx.
Abstract:
We describe the enzymatic synthesis of biodiesel from waste cooking oil (WCO) in a two-step production process: hydrolysis of WCO, followed by acid-catalyzed esterification of free fatty acids (FFAs). Among the three commercial enzymes evaluated, the inexpensive lipase Lipex® 100L supported on Lewatit® VP OC 1600 produced the best overall biodiesel yield (96.3%). Finally, we assessed the combustion efficiency of the obtained biodiesel and its blends. All blends tested presented lower emissions of CO and HC compared to diesel. The NOx emissions were higher due to biodiesel's high volatility and viscosity. The cost of biodiesel production was calculated using the process described.
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