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Establishing 3D Endometrial Organoids from the Mouse Uterus
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IL-22 regulates endometrial regeneration by enhancing tight junctions and orchestrating extracellular matrix
Umida Ganieva1, Sylvia Schneiderman2, Pengli Bu3
1Center for Cancer Cell Biology, Immunology, and Infection, Rosalind Franklin University of Medicine and Science, North Chicago, IL, United States.
Frontiers in Immunology
|September 12, 2022
Summary
Interleukin 22 (IL-22) is vital for uterine regeneration after abortion. IL-22 deficiency in mice led to secondary infertility and impaired endometrial repair, highlighting its therapeutic potential.
Area of Science:
- Reproductive Biology
- Immunology
- Cell Biology
Background:
- The uterine endometrium undergoes cyclical regeneration, crucial for female reproductive health.
- Endometrial integrity relies on cell-cell junctions, and disruptions can impact regeneration.
- Interleukin 22 (IL-22) is a cytokine known for its role in epithelial regeneration and mucosal immunity.
Purpose of the Study:
- To investigate the role of IL-22 in endometrial recovery following inflammation-induced abortion.
- To assess the impact of IL-22 deficiency on fertility and uterine healing in a mouse model.
Main Methods:
- Studied fecundity in consecutive matings of IL-22 knockout (IL-22-/-) and wild-type (WT) mice after lipopolysaccharide (LPS)-induced abortion.
- Evaluated uterine clearance and examined endometrial tissue for changes in tight junctions, mucin-1, and epithelial-mesenchymal transition markers.
Main Results:
- IL-22-/- mice exhibited significantly lower fecundity (9.1%) after the second mating compared to WT mice (71.4%), suggesting secondary infertility.
- IL-22 deficiency resulted in delayed uterine clearance of retained fetuses and reduced expression of tight junction proteins (claudin-2, claudin-10) and mucin-1.
- IL-22 influenced the remodeling of uterine tissue by regulating E- and N-cadherin expression during inflammation.
Conclusions:
- IL-22 is essential for proper endometrial regeneration and uterine tissue remodeling after inflammation-triggered abortion.
- IL-22 deficiency is linked to secondary infertility and impaired barrier function in the endometrium.
- IL-22 may hold therapeutic potential for enhancing postpartum regeneration and treating inflammation-induced secondary infertility.
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