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Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...

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Related Experiment Video

Updated: Aug 29, 2025

Murine Full-thickness Skin Transplantation

Murine Full-thickness Skin Transplantation

Published on: January 2, 2017

Two-stage skin grafting using a basic fibroblast growth factor-impregnated artificial dermis.

Ryushiro Sugimoto¹, Hiroki Yamanaka¹, Itaru Tsuge¹

¹Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, 54 Shogoin, Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan.

Regenerative Therapy

|September 12, 2022

Summary

Basic fibroblast growth factor (bFGF)-impregnated artificial dermis significantly shortens the waiting period for skin grafting after surgical reconstruction. This bFGF-impregnated collagen-gelatin sponge (CGS) approach shows high success rates and acceptable scar quality.

Keywords:

Artificial dermis Basic fibroblast growth factor CGS, collagen-gelatin sponge GCMN, giant congenital melanocytic nevus MRSA, methicillin-resistant Staphylococcus aureus Two-stage skin grafting VSS, Vancouver Scar Scale Waiting period bFGF, basic fibroblast growth factor

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Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair

Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair

Published on: February 24, 2016

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Last Updated: Aug 29, 2025

Murine Full-thickness Skin Transplantation

Murine Full-thickness Skin Transplantation

Published on: January 2, 2017

Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation

Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation

Published on: March 4, 2016

Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair

Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair

Published on: February 24, 2016

Area of Science:

Regenerative Medicine
Biomaterials Science
Plastic and Reconstructive Surgery

Background:

Traditional artificial dermises require a three-week waiting period for vascularization before secondary skin grafting.
This delay can impact patient recovery and treatment timelines in surgical reconstruction.
Optimizing wound healing and reducing treatment duration are key goals in reconstructive surgery.

Purpose of the Study:

To evaluate the efficacy of basic fibroblast growth factor (bFGF)-impregnated collagen-gelatin sponge (CGS) for treating full-thickness skin defects.
To determine if bFGF-impregnated CGS can shorten the waiting period for secondary skin grafting compared to traditional methods.
To assess the success rate, infection incidence, and scar quality following bFGF-CGS implantation and grafting.

Main Methods:

A retrospective case series involving 19 skin defects in 14 patients treated between January 2019 and January 2021.
Two-stage skin grafting utilizing bFGF-impregnated CGS as the artificial dermis.
Retrospective analysis of waiting period duration, skin graft success, infection rates, and scar quality (Vancouver Scar Scale).

Main Results:

All skin grafting surgeries were successful, with an average waiting period of 13.3 ± 4.3 days.
Infection occurred in 15.8% of lesions but was manageable in all cases.
Postoperative scar quality was generally acceptable, with Vancouver Scar Scale scores ranging from 1 to 8.

Conclusions:

bFGF-impregnated CGS offers a promising alternative to traditional artificial dermises for skin reconstruction.
This approach significantly reduces the waiting time for secondary skin grafting without compromising success rates.
Further research is warranted to validate these findings and explore broader clinical applications.