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Updated: Aug 29, 2025

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
A novel SERS substrate of MIL-100(Fe)/AgNFs for sensitive detection of ascorbic acid in cellular media
Wang Qiao1,2, Yiran Wang1,2, Zhenxia Zhao1
1School of Chemistry and Chemical Engineering, Guangxi Key Laboratory of Agro-Environment and Agro-Product Safety, Guangxi University Nanning 530004 China.
Abstract:
A novel SERS substrate of MIL-100(Fe)/AgNFs was firstly prepared for sensitive and selective detection of ascorbic acid (L-AA), with a LOD of 10-11 M. A spectral decrease of MIL-100(Fe)/AgNFs towards L-AA solution thanks to the efficient capture and reduction of Fe3+ in MIL-100(Fe) constituted the assay, which was demonstrated to function well in food samples and in cellular media for L-AA sensing.

