Related Experiment Videos
Chromatographic methods of fractionation
Developments in Biological Standardization
|January 1, 1987
Summary
Chromatography enables efficient, large-scale purification of plasma proteins like albumin and immune globulins. This advanced method ensures high-quality protein fractions for therapeutic use.
Area of Science:
- Biotechnology
- Chemical Engineering
- Biochemistry
Background:
- Chromatography is a versatile technique for industrial protein purification due to its efficiency and scalability.
- Plasma fractionation facilities utilize chromatography for large-scale purification of therapeutic proteins.
Purpose of the Study:
- To demonstrate the application of chromatography in a large-scale plasma fractionation facility.
- To highlight the purification of specific plasma proteins and human blood products using chromatographic methods.
Main Methods:
- Ion exchange chromatography using DEAE-Sepharose, DEAE-Biogel, and CM-Sepharose columns for albumin and i.v. ISG purification.
- Automated process control via a Modicon 584 Programmable Logic Controller for efficient plasma fractionation.
- Pilot-scale purification of superoxide dismutase and hemoglobin using DEAE-Sepharose and metal chelation.
Main Results:
- Successful large-scale purification of blood clotting factors, albumin, and intravenous immune serum globulin (i.v. ISG).
- Automated cleaning-in-place (CIP) systems ensure sanitary processing and efficient operation.
- Chromatographically purified protein fractions meet or exceed the quality standards of other purification techniques.
Conclusions:
- Chromatography is a highly effective and scalable method for industrial plasma protein purification.
- The automated fractionation process ensures high efficiency, sanitary conditions, and consistent product quality.
- Chromatographic techniques are suitable for purifying various human blood products, including those derived from outdated red blood cells.