RNA velocity unraveled
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Updated: Aug 29, 2025

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
Published on: February 12, 2022
Gennady Gorin1, Meichen Fang2, Tara Chari2
1Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California, United States of America.
Abstract:
We perform a thorough analysis of RNA velocity methods, with a view towards understanding the suitability of the various assumptions underlying popular implementations. In addition to providing a self-contained exposition of the underlying mathematics, we undertake simulations and perform controlled experiments on biological datasets to assess workflow sensitivity to parameter choices and underlying biology. Finally, we argue for a more rigorous approach to RNA velocity, and present a framework for Markovian analysis that points to directions for improvement and mitigation of current problems.
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