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Updated: Aug 29, 2025

Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates
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Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates

Published on: February 27, 2016

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Activation of SIRT1 promotes membrane resealing via cortactin

Naotoshi Iwahara1,2, Kuya Azekami1, Ryusuke Hosoda1

  • 1Department of Pharmacology, Sapporo Medical University School of Medicine, Sapporo, Japan.

Scientific Reports
|September 12, 2022
PubMed

Insights

Resveratrol enhances muscle membrane repair by activating SIRT1, which promotes cytoskeletal reorganization. This mechanism involves cortactin, offering a potential therapeutic avenue for muscular dystrophies.

Area of Science:

  • Muscle physiology and cellular repair mechanisms.
  • Investigating therapeutic compounds for inherited myopathies.

Background:

  • Muscular dystrophies cause progressive muscle weakness, with limited treatment options.
  • Resveratrol, a SIRT1 activator, shows potential in ameliorating muscular function, but its mechanism is unclear.

Purpose of the Study:

  • To investigate the effects of resveratrol on muscle membrane resealing.
  • To elucidate the molecular mechanisms underlying resveratrol-mediated membrane repair.

Main Methods:

  • Utilized C2C12 cells and single muscle fibers from mice for membrane repair assays.
  • Examined the role of SIRT1, actin, and cortactin in resveratrol-induced membrane repair.
  • Investigated SIRT1's effect on cortactin deacetylation and F-actin interaction.

Main Results:

  • Resveratrol promoted membrane repair in C2C12 cells and ex vivo muscle fibers via SIRT1 activation.
  • Resveratrol enhanced actin accumulation at injury sites.
  • SIRT1 deacetylated cortactin, promoting its interaction with F-actin, crucial for cytoskeletal reorganization and repair.

Conclusions:

  • Resveratrol facilitates muscle membrane repair through the SIRT1/cortactin pathway.
  • Targeting the SIRT1/cortactin axis may offer a novel therapeutic strategy for muscular dystrophies.

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