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Published on: February 20, 2012
Molecular characterization of Heterodera cruciferae Franklin, 1945 from cabbage fields in Niğde province, Turkey
Halil Toktay1, Badel G Akyol2, Emre Evlice3
1Department of Plant Production and Technologies, Faculty of Agricultural Sciences and Technologies, University of Nigde, Nigde, Turkey. h.toktay@ohu.edu.tr.
Background:
The aim of this study was to identified cyst nematodes in the cabbage production areas in Niğde Province by molecular methods to underpin decision making for field control. The sequences of ribosomal DNA region (rDNA-ITS) and cytochrome oxidase subunit 1 (mtDNA-COI) were used for the first time for identification Heterodera cruciferae (cabbage cyst nematode) in Turkey.
Methods And Results:
Heterodera cruciferae populations extracted from cabbage growing areas of Niğde Province and investigated using sequences of rDNA-ITS and mtDNA-COI. Similarities and differences between 13 geographic populations of H. cruciferae were detected in surveys during 2020-2021. DNA from single cysts was successfully amplified, and genetic variability was revealed within nematode populations. Based on these results, H. cruciferae is reported for the first time from Niğde Province, Turkey. This study showed clear discrimination among the sampled populations of H. cruciferae.
Conclusion:
This finding is important for control and managing cabbage cyst nematode in cabbage fields in Turkey as more than one Heterodera spp. can occur. Future studies should investigate the population dynamics and control of H. cruciferae in fields in the sampled districts.
