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Protocol to isolate and analyze mouse bone marrow derived dendritic cells (BMDC).

Manuela Sauter1, Reinhard J Sauter1, Henry Nording1

  • 1Department of Cardiology, University Hospital, Medical Clinic II, University Heart Center Luebeck, Ratzeburger Allee 160, 23538 Luebeck, Germany.

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|September 13, 2022
PubMed
Summary
This summary is machine-generated.

This study presents an in vitro method to analyze CD11c+ cells and their derived ApoE in atherosclerosis research. This adaptable protocol aids in studying immune cells and cell-specific molecules in various disease models.

Keywords:
Cell biologyCell cultureCell isolationFlow cytometry/Mass cytometryHealth sciencesImmunologyMolecular biology

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Area of Science:

  • Immunology
  • Cardiovascular Research
  • Cell Biology

Background:

  • Atherosclerosis involves diverse immune cells with atheroprotective or atheroprogressive roles.
  • Understanding specific immune cell contributions, like CD11c+ cells, is crucial for atherosclerosis research.

Purpose of the Study:

  • To describe an in vitro protocol for analyzing CD11c+ cells and CD11c+ derived ApoE in atherosclerosis.
  • To provide a adaptable model for studying immune cell functions and cell-specific molecules in various disease contexts.

Main Methods:

  • Harvesting mouse bone marrow.
  • Culturing cells and treating with differentiation factors.
  • Isolating specific cell populations, including CD11c+ cells.

Main Results:

  • The protocol enables the isolation and analysis of CD11c+ cells.
  • It facilitates the study of CD11c+ derived ApoE in the context of atherosclerosis.
  • The method is adaptable for different cell types and disease models.

Conclusions:

  • The described in vitro approach is valuable for dissecting the role of CD11c+ cells in atherosclerosis.
  • This protocol serves as a versatile tool for immunological and molecular studies in disease research.