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Updated: Aug 29, 2025

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
Published on: April 28, 2022
Molecular recognition of methylated amino acids and peptides by Pillar[6]MaxQ
David King1, Chelsea R Wilson2, Lukas Herron3,4
1Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742, USA. lisaacs@umd.edu.
Abstract:
We report the molecular recognition properties of Pillar[n]MaxQ (P[n]MQ) toward a series of (methylated) amino acids, amino acid amides, and post-translationally modified peptides by a combination of 1H NMR, isothermal titration calorimetry, indicator displacement assays, and molecular dynamics simulations. We find that P6MQ is a potent receptor for N-methylated amino acid side chains. P6MQ recognized the H3K4Me3 peptide with Kd = 16 nM in phosphate buffered saline.
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