CEG-AgNPs Ameliorates DMBA-Induced Mammary Carcinogenicity by Alleviating Cytokines Expression

Area of Science:

• Nanotechnology
• Biochemistry
• Oncology

Background:

• Breast cancer remains a leading global health concern for women.
• Evaluating the efficacy of novel therapeutic agents is crucial for cancer treatment.
• Carcinogenesis often involves complex molecular pathways requiring targeted interventions.

Purpose of the Study:

• To investigate the protective effects of green synthesized silver nanoparticles (CEG-AgNPs) against 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary carcinoma.
• To characterize the synthesized CEG-AgNPs and determine their safety profile (IC50 and LD50).
• To assess the impact of CEG-AgNPs on biochemical markers, oxidative stress, and gene expression in a mouse model.

Main Methods:

• Green synthesis and characterization of CEG-AgNPs.
• In vitro cytotoxicity testing (IC50) against MCF7 cell line and in vivo toxicity testing (LD50) in mice.
• Induction of mammary carcinoma in mice using DMBA, followed by treatment with CEG-AgNPs.
• Biochemical analysis of plasma and mammary tissue markers, including oxidative stress indicators and inflammatory cytokines.
• Evaluation of VEGF-C gene expression via RT-PCR.

Main Results:

• CEG-AgNPs were characterized with an average size of 42.32±9.52 nm and a negative zeta potential (-17.44).
• The IC50 against MCF7 cells was 82.76 μg/mL, and the LD50 in mice was 1405 mg/kg b.wt.
• CEG-AgNPs treatment normalized plasma enzymes (ALT, AST, LDH) and mammary tissue markers (MDA, TNF-α, IL-6, P53, SOD, GPx, GSH).
• Oral administration of CEG-AgNPs significantly suppressed VEGF-C gene expression in DMBA-treated mice.

Conclusions:

• CEG-AgNPs exhibit potent anticancer activity against DMBA-induced mammary carcinoma in a murine model.
• The therapeutic effect is attributed to the induction of antioxidant biomarkers and suppression of pro-inflammatory cytokine gene expression.
• CEG-AgNPs represent a promising therapeutic candidate for breast cancer treatment, supported by biochemical, histological, and MRI findings.