Nanocarriers with Multiple Cargo Load-A Comprehensive Preparation Guideline Using Orthogonal Strategies

Area of Science:

• Biomedical Engineering
• Materials Science
• Drug Delivery

Background:

• Multifunctional nanocarriers are crucial for enhancing therapeutic efficacy.
• Coencapsulation of multiple bioactive molecules enables synergistic therapies.
• Challenges exist in coencapsulating diverse molecules due to differing physicochemical properties.

Purpose of the Study:

• To provide a guideline for selecting suitable preparation protocols for nanocarrier systems.
• To facilitate the selection of cargo molecules (hydrophilic-hydrophobic, small-macro, organic-inorganic) for coencapsulation.
• To focus on coencapsulating multiple cargo molecules into biocompatible and biodegradable nanocarriers using orthogonal strategies.

Main Methods:

• Review of orthogonal preparation strategies for nanocarriers.
• Discussion of physical processes (self-assembly, coacervation) and chemical reactions (click chemistry, polymerization).
• Emphasis on methods preserving bioactivity of sensitive encapsulated molecules.

Main Results:

• Orthogonal approaches are essential for controlled and efficient coencapsulation.
• Selection of appropriate synthesis protocols based on cargo properties and desired nanocarrier characteristics.
• Development of a toolbox for preparing biodegradable nanocarriers loaded with multiple cargo molecules.

Conclusions:

• Orthogonal strategies offer a robust method for creating multifunctional nanocarriers.
• This review empowers researchers to design advanced nanocarrier systems for synergistic drug delivery.
• The presented framework supports the development of tailored nanocarriers for diverse therapeutic applications.