Broad-Spectrum Antibacterial Activity of Synthesized Carbon Nanodots from d-Glucose

Carbon nanodots, a class of carbon nano-allotropes, have been synthesized through different routes and methods from a wide range of precursors. The selected precursor, synthetic method, and conditions can strongly alter the physicochemical properties of the resulting material and their intended applications. Herein, carbon nanodots (CNDs) have been synthesized from d-glucose by combining pyrolysis and chemical oxidation methods. The effect of the pyrolysis temperature, equivalents of oxidizing agent, and refluxing time were studied on the product and quantum yield. In the optimum conditions (pyrolysis temperature of 300 °C, 4.41 equiv of H₂O₂, 90 min of reflux) CNDs were obtained with 40% and 3.6% of product and quantum yields, respectively. The obtained CNDs are negatively charged (ζ-potential = -32 mV), excellently dispersed in water, with average diameter of 2.2 nm. Furthermore, ammonium hydroxide (NH₄OH) was introduced as dehydrating and/or passivation agent during CNDs synthesis resulting in significant improvement of both product and quantum yields of about 1.5 and 3.76-fold, respectively. The synthesized CNDs showed a broad spectrum of antibacterial activities toward different Gram-positive and Gram-negative bacteria strains. Both synthesized CNDs caused highly colony forming unit reduction (CFU), ranging from 98% to 99.99% for most of the tested bacterial strains. However, CNDs synthesized in the absence of NH₄OH, due to a negatively charged surface enriched in oxygenated groups, performed better in zone inhibition and minimum inhibitory concentration. The elevated antibacterial activity of high-oxygen-containing carbon nanodots is directly correlated to their ROS formation ability.