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Updated: Aug 28, 2025

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
Published on: March 6, 2018
Optical quantum super-resolution imaging and hypothesis testing
Ugo Zanforlin1, Cosmo Lupo2, Peter W R Connolly3
1Scottish Universities Physics Alliance, Institute of Photonics and Quantum Sciences, School of Engineering and Physical Sciences, Heriot-Watt University, David Brewster Building, Edinburgh, EH14 4AS, UK. U.Zanforlin@hw.ac.uk.
Abstract:
Estimating the angular separation between two incoherent thermal sources is a challenging task for direct imaging, especially at lengths within the diffraction limit. Moreover, detecting the presence of multiple sources of different brightness is an even more severe challenge. We experimentally demonstrate two tasks for super-resolution imaging based on hypothesis testing and quantum metrology techniques. We can significantly reduce the error probability for detecting a weak secondary source, even for small separations. We reduce the experimental complexity to a simple interferometer: we show (1) our set-up is optimal for the state discrimination task, and (2) if the two sources are equally bright, then this measurement can super-resolve their angular separation. Using a collection baseline of 5.3 mm, we resolve the angular separation of two sources placed 15 μm apart at a distance of 1.0 m with a 1.7% accuracy - an almost 3-orders-of-magnitude improvement over shot-noise limited direct imaging.
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