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Updated: Aug 28, 2025

Induction and Assessment of Class Switch Recombination in Purified Murine B Cells
Published on: August 13, 2010
Transcriptional regulation of B cell class-switch recombination: the role in development of noninfectious
Stelios Vlachiotis1, Hassan Abolhassani1,2
1Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.
Introduction:
The process of immunoglobulin class switch recombination (CSR) occurs in secondary lymphoid organs. This highly regulated process is essential for the development of different antibody isotype maturation and long-life memory/plasma cell generation. Patients with impaired CSR present heterogeneous noninfectious complications.
Areas Covered:
We provide an overview of recent advancements in the tight regulation of B cells before and during the CSR at different levels of cytokine stimulations, intracellular signaling, transcription-factor activation, gene transcription, and epigenetic controls.
Expert Opinion:
Besides recurrent infections which result from the lack of production of class-switched immunoglobulins, intrinsic B cell signaling pathways and regulatory component defects have distinct roles in other immune-related clinical manifestations including autoimmunity, atopy, lymphoproliferation, and cancer.
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