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Resistance to Paracoccidioides brasiliensis in mice is controlled by a single dominant autosomal gene
Abstract:
In a previous report it was shown that there are resistant, susceptible, and intermediate strains of mice to intraperitoneal Paracoccidioides brasiliensis infection. In the present work, we investigated the type of inheritance and the number of genes that determine resistance to paracoccidioidomycosis. Parental and hybrid mice were inoculated intraperitoneally with 5 X 10(6) P. brasiliensis yeast cells, and mortality was scored daily. Analysis of susceptible and resistant parental strains and of F1, F2, and backcross mice showed that the resistance to P. brasiliensis seems to be controlled genetically by a single dominant gene, which we designated the Pbr locus. The mean survival times of susceptible F2 and backcross hybrids were very similar to that of the susceptible parent. Examination of the pathological changes observed in parental and F1 mice, 6 months after infection, showed that F1 offspring presented a similar number and distribution of lesions to those of the resistant strains. The Pbr gene is not linked to H-2, Hc, and albino genes. Furthermore, resistance to paracoccidioidomycosis is controlled by an autosomal gene.
Insights
Genetic resistance to Paracoccidioides brasiliensis infection in mice is controlled by a single dominant gene, Pbr. This finding advances understanding of paracoccidioidomycosis inheritance patterns.
Area of Science:
- Immunology
- Genetics
- Infectious Diseases
Background:
- Previous studies identified varying mouse strain susceptibility to Paracoccidioides brasiliensis.
- Understanding the genetic basis of this resistance is crucial for disease management.
Purpose of the Study:
- To investigate the inheritance pattern and number of genes controlling resistance to paracoccidioidomycosis.
- To identify the genetic locus responsible for resistance.
Main Methods:
- Inoculation of parental, F1, F2, and backcross mice with P. brasiliensis.
- Daily mortality scoring and pathological examination of infected mice.
- Genetic linkage analysis with known mouse genes (H-2, Hc, albino).
Main Results:
- Resistance to P. brasiliensis is controlled by a single dominant autosomal gene, designated Pbr.
- F1 offspring exhibited resistance similar to resistant parental strains.
- Susceptible F2 and backcross hybrids showed survival times comparable to susceptible parents.
- The Pbr gene is not linked to H-2, Hc, or albino genes.
Conclusions:
- A single dominant gene (Pbr) dictates mouse resistance to P. brasiliensis.
- This genetic control is autosomal and independent of H-2, Hc, and albino loci.
- The Pbr locus provides a key target for understanding and potentially manipulating paracoccidioidomycosis resistance.