Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Caught in the crossfire: cardiac complications of cancer therapy.

The Journal of clinical investigation·2026
Same author

ATP-binding cassette B8 prevents endothelial dysfunction and atherosclerosis.

Redox biology·2025
Same author

Iron Metabolism in Cardiovascular Disease.

Advances in experimental medicine and biology·2025
Same author

The Impact of the COVID-19 Pandemic on Physical, Social and Emotional Well-Being in a Cohort of Children With Disabilities in the United States.

Journal of paediatrics and child health·2025
Same author

Protein O-GlcNAcylation and hexokinase mitochondrial dissociation drive heart failure with preserved ejection fraction.

Cell metabolism·2025
Same author

Human induced pluripotent stem cell-derived cardiomyocytes to study inflammation-induced aberrant calcium transient.

eLife·2024

Related Experiment Video

Updated: Aug 28, 2025

Author Spotlight: Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification
09:04

Author Spotlight: Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification

Published on: June 2, 2023

6.4K

Optimized protocol to isolate primary mouse peritoneal macrophage metabolites.

Adam De Jesus1, Carolina M Pusec2, Tivoli Nguyen1

  • 1Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA; Feinberg Cardiovascular Research Institute, Northwestern University, Chicago, IL 60611, USA.

STAR Protocols
|September 14, 2022
PubMed
Summary

This study presents a new protocol for extracting metabolites from low numbers of primary peritoneal macrophages (PMs). This method enables crucial metabolomics analysis and stable isotope tracing in immune cells.

Keywords:
Cell isolationImmunologyMass spectrometryMetabolismMetabolomics

More Related Videos

Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation
08:21

Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation

Published on: April 29, 2015

36.8K
Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages
07:24

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Published on: October 19, 2018

14.6K

Related Experiment Videos

Last Updated: Aug 28, 2025

Author Spotlight: Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification
09:04

Author Spotlight: Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification

Published on: June 2, 2023

6.4K
Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation
08:21

Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation

Published on: April 29, 2015

36.8K
Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages
07:24

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Published on: October 19, 2018

14.6K

Area of Science:

  • Immunology
  • Metabolomics
  • Cell Biology

Background:

  • Peritoneal macrophages (PMs) exhibit superior stability compared to other macrophage subtypes.
  • Limited cell numbers from single mice pose challenges for metabolomics analysis of PMs.

Purpose of the Study:

  • To develop an efficient protocol for isolating metabolites from small quantities of mouse primary PMs.
  • To facilitate 13C-stable glucose tracing and metabolomics analysis in low-cell-number PM samples.

Main Methods:

  • A novel metabolite extraction protocol utilizing compound X, replacing traditional methanol-based methods.
  • Application of the protocol to low cell number samples of primary mouse PMs.
  • 13C-stable glucose tracing for metabolic pathway analysis.

Main Results:

  • Consistent metabolite extraction from low cell number samples.
  • Fewer procedural steps compared to conventional methanol-based extraction techniques.
  • Successful application in metabolomics and stable isotope tracing.

Conclusions:

  • The developed protocol effectively overcomes cell number limitations for PM metabolomics.
  • This method provides a streamlined approach for analyzing macrophage metabolism.
  • Enables advanced analyses like stable isotope tracing in scarce immune cell populations.