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Updated: Aug 28, 2025
Bioluminescence Imaging to Detect Late Stage Infection of African Trypanosomiasis
Published on: May 18, 2016
Protocol to analyze the transmigration efficiency of T. brucei using an in vitro model of the blood-cerebrospinal
Alexander Herrmann1, Carolin Susanne Schnedermann1, Hiroshi Ishikawa2
1Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany.
Abstract:
At present, the only approach to investigate the transmigration of Trypanosoma brucei, the causative agent of human African trypanosomiasis, from blood to cerebrospinal fluid is through animal experiments. This protocol details how to analyze the transmigration efficiency using an in vitro model of the blood-cerebrospinal fluid (blood-CSF) barrier. We describe how to grow human choroid plexus epithelial cells on cell culture filter inserts to form the barrier, followed by isolating and quantifying genomic DNA of transmigrated parasites by qPCR. For complete details on the use and execution of this protocol, please refer to Speidel et al. (2022).
