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Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
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Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Published on: December 31, 2017

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Characteristics of salivary microbiota in children with obstructive sleep apnea: A prospective study with

Xin Huang1, Xuehui Chen1, Xu Gong1

  • 1Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing, China.

Frontiers in Cellular and Infection Microbiology
|September 15, 2022
PubMed

Insights

Children with obstructive sleep apnea (OSA) have altered salivary microbiota, with higher diversity and specific bacterial genera like Prevotella. These changes normalize after adenotonsillectomy, suggesting a link between OSA and oral microbiome.

Area of Science:

  • Microbiology
  • Pediatric Sleep Medicine
  • Bioinformatics

Background:

  • Obstructive sleep apnea (OSA) is a common pediatric condition affecting sleep quality and potentially oral health.
  • The oral microbiome plays a crucial role in maintaining oral health and can be influenced by various physiological conditions.

Purpose of the Study:

  • To characterize the salivary microbiota in children diagnosed with obstructive sleep apnea (OSA).
  • To investigate longitudinal changes in salivary microbiota composition before and after adenotonsillectomy in OSA patients.

Main Methods:

  • Cross-sectional study comparing salivary microbiota of 36 OSA children and 22 controls using 16S rRNA sequencing.
  • Longitudinal analysis of 8 OSA children before and 1 year after adenotonsillectomy.
  • Bioinformatic analyses including diversity metrics (α- and β-diversity), random forest classification, and LEfSe for differential abundance.

Main Results:

  • OSA children exhibited significantly higher α-diversity and distinct β-diversity compared to controls.
  • Key genera differentiating OSA from controls included Lactobacillus, Escherichia, Bifidobacterium, and Prevotella.
  • Prevotella, particularly Prevotella sp. HMT 396, was enriched in OSA patients, with abundance correlating positively with OSA severity and decreasing post-adenotonsillectomy.

Conclusions:

  • Salivary microbiota in children with OSA is characterized by increased diversity and altered composition, notably with higher Prevotella abundance.
  • Adenotonsillectomy appears to modulate the salivary microbiome towards a profile closer to non-OSA children.
  • The findings highlight a potential role for Prevotella in pediatric OSA and suggest salivary microbiota as a biomarker.
Abstract

Keywords:
16S rRNA gene sequencingPediatric obstructive sleep apneaadenotonsillectomypolysomnographysalivary microbiota

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